Furthermore, 3-nitrobenzoic acid has also been reported as a potent CAI, with further studies showing its potential clinical relevance as a cancer therapeutic. Unlike the sulfonamide-based drugs, these inhibitors do not directly displace the zinc bound solvent, but instead anchor through the solvent, blocking substrate entry to the active site. Nicotinic and ferulic acid have recently been identified as inhibitors of CAII. In addition to sulfonamides, a variety of other chemical motifs have been identified to inhibit CA, such as carboxylic acids. Many of these sulfonamide-based molecules are used clinically, such as dorzolamide, for the treatment of glaucoma. The most common type of CAIs are sulfonamides, such as acetazolamide, which has nM binding affinity. These inhibitors are designed to bind to the active site zinc, displacing the zinc bound solvent. In addition, CAIs are currently being developed as anti-cancer drugs. CA inhibitors (CAIs) are used to treat a variety of diseases such as glaucoma, altitude sickness, and epilepsy.
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